Examination of Biomaterial Samples Obtained from Coffee and Tangerine Plants at Nepal’s Plantations Affected by Phytopathogens and Determination of The Efficiency of Their Suppression by The Biofungicide

Keywords: Biopreparations, phytopathogen, plants, plant protection, rot

Infestation of plants with phytopathogenic infections brings great harm to agriculture. This is a global problem. Entire regions lose their business of growing coffee, date palms, various fruits and vegetables, as well as grain crops, fruit trees, shrubs and flowers. These phenomena are especially manifested in places where chemical pesticides, insecticides and herbicides are used in large amounts, where soils are depleted and where there is a lack of moisture. First of all, the weakened plants are susceptible to various diseases due to reduced immunity. This work examines biomaterial samples from infected coffee and tangerine plants taken from Nepal's plantations. Examination was conducted in the BIOIN-NOVO LTD laboratory. The isolated pathogens were purified, their biomass was built up, and molecular genetic examination was performed, the genus and species of the phytopathogens were determined. A collection of biofungicides based on the fungi of Trichoderma genus available at the company was screened, revealing the possibility of efficient suppression of phytopathogens by various strains. An experimental sample of a complex biopreparation was obtained from the most active strains using biotechnology techniques in order to test its efficiency under the conditions of these plants plantation